Difference between revisions of "Part:BBa K2984035"
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| − | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containing <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, fused to <a href="https://parts.igem.org/wiki/index.php?title=Part: | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containing <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, fused to <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984024">mVenus</a> fluorescent marker for fluorecence meausrements of PETase concentration and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984014">His-tag</a> for purification. The parts are flanked by the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984021">Rbcs2 terminator</a>. The part has a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a> connecting the PsaD promoter and the PEtase. |
The construct is desgined to measure the expression level of PETase. | The construct is desgined to measure the expression level of PETase. | ||
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Latest revision as of 13:05, 17 October 2019
L1c-Psad-PETase-YFP-His-RbcS2
This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containing PETase enzyme, fused to mVenus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the PsaD promoter and the Rbcs2 terminator. The part has a B2-B2 linker connecting the PsaD promoter and the PEtase. The construct is desgined to measure the expression level of PETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1705
Illegal PstI site found at 1919 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1244
Illegal PstI site found at 1705
Illegal PstI site found at 1919 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 968 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1705
Illegal PstI site found at 1919 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1705
Illegal PstI site found at 1919
Illegal NgoMIV site found at 2396 - 1000COMPATIBLE WITH RFC[1000]
Characterization
