Difference between revisions of "Part:BBa K2984052"
Line 5: Line 5:
 
<html>
 
<html>
 
This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 construct containing secretion signal of arylsulfatase <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> fused to the flourescent protein <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a>. To influence the expression of mVenus, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984045">bleomycin resistance</a> gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984046">PsaDintron</a>. The construct ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>.
 
This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 construct containing secretion signal of arylsulfatase <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> fused to the flourescent protein <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a>. To influence the expression of mVenus, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984045">bleomycin resistance</a> gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984046">PsaDintron</a>. The construct ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>.
 +
</html>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:46, 17 October 2019


L1c-PsadIntron-bleRscp-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron. The construct ends with the Rbcs2 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2051
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2051
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2051
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2051
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 2528
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii