Difference between revisions of "Part:BBa K2984052"
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<partinfo>BBa_K2984052 short</partinfo> | <partinfo>BBa_K2984052 short</partinfo> | ||
| − | Level 1 construct containing secretion signal of arylsulfatase fused to the flourescent protein mVenus. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the | + | <html> |
| + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 construct containing secretion signal of arylsulfatase <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> fused to the flourescent protein <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a>. To influence the expression of mVenus, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984045">bleomycin resistance</a> gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984046">PsaDintron</a>. The construct ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2984052 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984052 SequenceAndFeatures</partinfo> | ||
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| + | ==Characterization== | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://2019.igem.org/wiki/images/f/fb/T--Humboldt_Berlin--Konstruk_16.jpg" alt="colonies_total" width="500"> | ||
| + | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
Revision as of 12:46, 17 October 2019
L1c-PsadIntron-bleRscp-ARS-YFP-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. Level 1 construct containing secretion signal of arylsulfatase ARS fused to the flourescent protein mVenus. To influence the expression of mVenus, a bleomycin resistance gene is connected through a self cleaving peptide (scp) to the part. The Promoter is the PsaDintron. The construct ends with the Rbcs2 terminator.
Sequence and Features
