Difference between revisions of "Part:BBa K2771000"
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Plux is a promoter which responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). In order to test this promoter function. We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for this part K2771000, thus making a valuable characterization for this part. | Plux is a promoter which responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). In order to test this promoter function. We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for this part K2771000, thus making a valuable characterization for this part. | ||
| + | ===Experiment Design and Measurement Methods=== | ||
| + | |||
| + | We cloned LuxR, lux promoter and GFP into psB1C3 vector, and constructed ppSB1C3-LuxR-Plux-GFP plasmid. gene circuit as Fig1.: | ||
Revision as of 11:40, 17 October 2019
Modified pLux
Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.
Usage and Biology
XHD-WS-Wuhan-A characterization
We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for part K2771000, thus making a valuable characterization for this part.
Plux is a promoter which responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). In order to test this promoter function. We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for this part K2771000, thus making a valuable characterization for this part.
Experiment Design and Measurement Methods
We cloned LuxR, lux promoter and GFP into psB1C3 vector, and constructed ppSB1C3-LuxR-Plux-GFP plasmid. gene circuit as Fig1.:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]