Difference between revisions of "Part:BBa K2771000"
Line 10: Line 10:
 
===Usage and Biology===
 
===Usage and Biology===
  
−
The expression of miR-196a  was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.
+
 
−
 
+
  
 
<!-- -->
 
<!-- -->
Line 21: Line 20:
 
===Functional Parameters===
 
===Functional Parameters===
  
−
The expression of miR-196a  was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.
+
 
 
<partinfo>BBa_K2771000 parameters</partinfo>
 
<partinfo>BBa_K2771000 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 11:35, 17 October 2019


Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.



Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters