Difference between revisions of "Part:BBa K2771000"
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===Functional Parameters=== | ===Functional Parameters=== | ||
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| + | The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor. | ||
<partinfo>BBa_K2771000 parameters</partinfo> | <partinfo>BBa_K2771000 parameters</partinfo> | ||
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Revision as of 11:29, 17 October 2019
Modified pLux
Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.
Usage and Biology
The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.