Difference between revisions of "Part:BBa K2771000"
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Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.
 
Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.
  
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The expression of miR-196a  was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.
 
  
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===Usage and Biology===
 
===Usage and Biology===
  
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The expression of miR-196a  was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.  
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                <p>the U.S. Centers for Disease Control and Prevention</p >
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Revision as of 11:27, 17 October 2019


Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.



Usage and Biology

The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]