Difference between revisions of "Part:BBa K678004"

 
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Promoter for expression of genes in mammalian cells
 
Promoter for expression of genes in mammalian cells
  
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===Contribution: NUDT_CHINA 2019===
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===Usage and Biology===
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Summary：in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours.
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Revision as of 11:04, 17 October 2019


PGK, mammalian promoter

Promoter for expression of genes in mammalian cells

Contribution: NUDT_CHINA 2019

Summary：in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 143
    Illegal PstI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]