Difference between revisions of "Part:BBa K2771000"

Revision as of 10:51, 17 October 2019

Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.

The expression of miR-196a was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.
+The expression of miR-196a  was found to be significantly associated with early tumor stage of gastric cancer. We designed miR-196a sensor contain two complementary binding sites to miR-196a, which inhibit miR-196a expression. The sequence of miR-196a sensor was synthetised by GenScript (Shanghai, China). We inserted miR-196a sensor into the 3' end of GFP, which generated GFP- miR-196a sensor.
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