Difference between revisions of "Part:BBa K2771000"
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===Usage and Biology===
 
===Usage and Biology===
  
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  <p>We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for part K2771000, thus making a valuable characterization for this part.
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Plux is a promoter which responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). In order to test this promoter function. We constructed a gene Gene circuit : LuxR-Plux-GFP to measure the function of promoter Plux under GFP protein. Based on our experiments, we have provided detailed data for this part K2771000, thus making a valuable characterization for this part.</p >
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                <p>the U.S. Centers for Disease Control and Prevention</p >
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https://static.igem.org/mediawiki/parts/4/43/T--XHD-WS-Wuhan-A--196a_fig_1.jpeg
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Revision as of 10:49, 17 October 2019


Modified pLux

Quorum sensing activated promoter that responds natively to the Lux pathway (activated by LuxR + 3OC6HSL). This specific instance of this promoter has a mutation on nucleotide 18 of the consensus sequence, which reportedly diminishes genetic crosstalk with the Las system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]