Difference between revisions of "Part:BBa K2984047"

 
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<partinfo>BBa_K2984047 short</partinfo>
 
<partinfo>BBa_K2984047 short</partinfo>
  
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This part is composed of the AR promoter (BBa_K2984009), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP-mVenus marker (BBa_K2984020) and the Rbcs2 terminator (BBa_K2984018). The part can be used to express PETase enzyme with a YFP marker in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.  
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This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984011">PETase enzyme</a>, a YFP <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984020">mVenus</a> marker and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express PETase enzyme with a YFP marker in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.  
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Revision as of 10:46, 17 October 2019


L1c-AR-PETase-YFP-RbcS2


This vector is a part of the Chlamy-HUB-Collection. This part is composed of the AR promoter, a B2-B2 linker, the PETase enzyme, a YFP mVenus marker and the Rbcs2 terminator. The part can be used to express PETase enzyme with a YFP marker in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1758
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 268
    Illegal NheI site found at 1087
    Illegal PstI site found at 1758
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 811
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1758
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1758
    Illegal NgoMIV site found at 2235
  • 1000
    COMPATIBLE WITH RFC[1000]