Difference between revisions of "Part:BBa K3160009:Design"
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The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a.
 
The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a.
  
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https://parts.igem.org/File:T--XHD-WS-Wuhan-A--196a_fig_1.jpeg
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                < img src="https://parts.igem.org/File:T--XHD-WS-Wuhan-A--196a_fig_1.jpeg">
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                <p>the U.S. Centers for Disease Control and Prevention</p >
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===Source===
 
===Source===

Revision as of 10:24, 17 October 2019


pEGFP-miR-196a sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a. 

               < img src="https://parts.igem.org/File:T--XHD-WS-Wuhan-A--196a_fig_1.jpeg">

the U.S. Centers for Disease Control and Prevention


Source

We ordered this part DNA from a synthesis company

References

1. Zheng G, Xiong Y, Xu W, Wang Y, Chen F, Wang Z, Yan Z. A two-microRNA signature as a potential biomarker for early gastric cancer. Oncol Lett. 2014 Mar;7(3):679-684.