Difference between revisions of "Part:BBa K3160009:Design"
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The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a. | The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a. | ||
| − | + | https://parts.igem.org/File:T--XHD-WS-Wuhan-A--196a_fig_1.jpeg | |
===Source=== | ===Source=== | ||
Revision as of 10:13, 17 October 2019
pEGFP-miR-196a sensor
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pEGFP-miR-196a sensorsensor was controlled by CMV promoter (CMV-GFP- miR-196a). The plasmids of CMV-GFP- miR-196a were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We also measuresd the fluorescence of GFP in different cells transfected with CMV-GFP- miR-196a for 24 h by plate reader (SpectraMax i3), which analyses the potential value of CMV-GFP- miR-196a to measure the expression of miR-196a.
https://parts.igem.org/File:T--XHD-WS-Wuhan-A--196a_fig_1.jpeg
Source
We ordered this part DNA from a synthesis company
References
1. Zheng G, Xiong Y, Xu W, Wang Y, Chen F, Wang Z, Yan Z. A two-microRNA signature as a potential biomarker for early gastric cancer. Oncol Lett. 2014 Mar;7(3):679-684.