Difference between revisions of "Part:BBa K2984029"
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<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | ||
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Revision as of 09:10, 17 October 2019
L1c-PsaD-GLE-PETase-YFP-SP20-RBCS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, the GLE secretion signal, the PETase enzyme, a YFP mVenus marker, the SP20 glycomodule for enhanced secretion and the Rbcs2 terminator (BBa_K29840). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression and secretion of the PETase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1314
Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
Illegal XhoI site found at 1038 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1775
Illegal PstI site found at 1989 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1775
Illegal PstI site found at 1989
Illegal NgoMIV site found at 2466 - 1000COMPATIBLE WITH RFC[1000]
Characterization
