Difference between revisions of "Part:BBa K3286041"
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(*We have observed that insertion of two spacers (with a repeat in the middle) is also possible with the use of ultramers.) | (*We have observed that insertion of two spacers (with a repeat in the middle) is also possible with the use of ultramers.) | ||
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<li>Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038</li> | <li>Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038</li> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Latest revision as of 08:47, 17 October 2019
F. novicida Cpf1 (Cas12a) CRISPR array
Francisella novicida Cpf1 (Cas12a) CRISPR array .
The Cpf1 (Cas12a) CRISPR array of F. novicida, with two repeats and a scrambled (non-targeting) spacer.
New spacers can be generated by BbsI digestion of the CRISPR array and ligation of two annealed oligo's (with 5'-AGAT... and 5'-AGAC overhangs). By doing so, we can generate very fast, cheap and reliable a library of CRISPR arrays*.
(*We have observed that insertion of two spacers (with a repeat in the middle) is also possible with the use of ultramers.)
- Zetsche, B., Gootenberg, J. S., Abudayyeh, O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., … Zhang, F. (2015). Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Cell, 163(3), 759–771. https://doi.org/10.1016/j.cell.2015.09.038
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]