Difference between revisions of "Part:BBa K3182300"
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<h1>Usage and Biology</h1> | <h1>Usage and Biology</h1> | ||
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[[File:T--Linkoping_Sweden--mngspectra.png|420px|thumb|left|<b>Figure 2.</b> ]] | [[File:T--Linkoping_Sweden--mngspectra.png|420px|thumb|left|<b>Figure 2.</b> ]] | ||
− | [[File:T--Linkoping_Sweden--mgnen.png|420px|thumb|left|<b>Figure 2.</b> ]] | + | [[File:T--Linkoping_Sweden--mgnen.png|420px|thumb|left|<b>Figure 2.</b>Comparison of the old biobrick, X (light grey), and the new biobrick BBa K3182300 (dark grey). Relative fluorescence after 16 hours is shown. The error bars represent the mean ± SD from four technical replicates.]] |
+ | To evenly compare the parts, both biobricks were grown to a starting optical density at 600 nm (OD600) of 0.4. | ||
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Revision as of 05:20, 17 October 2019
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 91
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Introduction
pT7-mNG
This is a improved variant of BBa_K2671000. The biobrick codes for mNeonGreen (mNG), which is a fluorescent protein with great intensity. The protein is currently ranked third in intensity, only beaten by mVenus-Q69M (basic) and Skylan-S (photoswitchable).
This part has a T7-system as well as a 5’-UTR region, instead of the AraC-pBAD system present in the non-improved biobrick BBa_K2671000. By using the T7-DNA-directed-RNA-polymerase (T7-DdRNAP) from a bacteriophage over the native DNA-directed-RNA-polymerase (n-DdRNAP) native in E. coli the expression is greatly increased. This part requires a host carrying the T7-DdRNAP, such as E. coli BL21 (DE3) Gold which was the chassi we used.
Usage and Biology
To evenly compare the parts, both biobricks were grown to a starting optical density at 600 nm (OD600) of 0.4.