Difference between revisions of "Part:BBa K3093100"

Revision as of 04:27, 17 October 2019

PlacIq-LacI-Plac-cI-λPR dual inverter

The lacI gene is expressed from an unregulated promoter Placlq, and its product LacI represses the Plac promoter. Likewise, the Plac promoter controls the expression of the cI gene, and its product CI represses the PR promoter. The PR promoter controls the production of the red fluorescent protein (mRFP1), which represents the‘output’for the circuit, whereas the chemical inducer isopropyl β-D-thiogalactopyranoside (IPTG), which binds to LacI tetramers and renders them unable to repress Plac, provides an external control over the network and thus represents the‘input.’

The inverter system consists of lactose operon, CⅠprotein and PλR012. The LacI repressor is constitutively expressed which inhibites transcription from the pLac promoter in the absence of IPTG, thus PλR012 could be active. While the expression of the CI repressor can be induced by externally added IPTG and inhibited the transcription of PλR012, hence realizing the reversal of the function.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1229
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 3156
Illegal AgeI site found at 3268
1000
COMPATIBLE WITH RFC[1000]

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	The lacI gene is expressed from an unregulated promoter Placlq, and its product LacI represses the Plac promoter. Likewise, the Plac promoter controls the expression of the cI gene, and its product CI represses the PR promoter. The PR promoter controls the production of the red fluorescent protein (mRFP1), which represents the‘output’for the circuit, whereas the chemical inducer isopropyl β-D-thiogalactopyranoside (IPTG), which binds to LacI tetramers and renders them unable to repress Plac, provides an external control over the network and thus represents the‘input.’		The lacI gene is expressed from an unregulated promoter Placlq, and its product LacI represses the Plac promoter. Likewise, the Plac promoter controls the expression of the cI gene, and its product CI represses the PR promoter. The PR promoter controls the production of the red fluorescent protein (mRFP1), which represents the‘output’for the circuit, whereas the chemical inducer isopropyl β-D-thiogalactopyranoside (IPTG), which binds to LacI tetramers and renders them unable to repress Plac, provides an external control over the network and thus represents the‘input.’