Difference between revisions of "Part:BBa K2957099"

Latest revision as of 03:41, 17 October 2019

IL-8 (CXCL-8) with FLAG Tag

Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance

What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression

What does it do?: It codes for the chemokine, IL-8 (CXCL8), which is involved in an inflammatory response. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming. It was important for our project in particular because of its involvement in the immune system and is known to create neutrophil swarming.

How to use it?: This part was used for Type IIS Cloning and transfected into HEKs. We then were able to test for secretion of IL8. See more on the Parts Overview Page or MIT iGEM 2019 wiki: <a href=https://2019.igem.org/Team:MIT/Parts>. "

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 244
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
-<partinfo>BBa_K2957003 short</partinfo>
+<partinfo>BBa_K2957001 short</partinfo>
-"Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance
+Contents: hEF1a, IL-8 (CXCL-8), SynthpA, with Carb resistance
 What is it?: A Composite part; it is a genetic circuit with CMV promoter and IL-8 gene allowing for constitutive IL-8 expression
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 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K2957003 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K2957001 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K2957003 parameters</partinfo>
+<partinfo>BBa_K2957001 parameters</partinfo>
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