Difference between revisions of "Part:BBa K3098021"
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But we found that, this promoter can be used not only for its osmosensing function, in some other condition, it is also be used as a part of a system, such as the red light system ( from Part: BBa_K1017301). In such condition, we hope to get rid of the influence of the osmosis, thus, we create a new part by adding a riboswitch after the promoter, to realize another kind of control via theophylline. | But we found that, this promoter can be used not only for its osmosensing function, in some other condition, it is also be used as a part of a system, such as the red light system ( from Part: BBa_K1017301). In such condition, we hope to get rid of the influence of the osmosis, thus, we create a new part by adding a riboswitch after the promoter, to realize another kind of control via theophylline. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This part better to be expressed in DH5α, because the EnvZ-OmpR is a protein naturally expressed in K-12 strain. And the unprocessed state of this part is off, because when there are id no theophylline, the riboswitch is turned off. When you want it to be on and start to express the target gene, you have to add the theophylline to open the riboswitch. Thus we achieved the tightly control of this promoter via a riboswitch. | This part better to be expressed in DH5α, because the EnvZ-OmpR is a protein naturally expressed in K-12 strain. And the unprocessed state of this part is off, because when there are id no theophylline, the riboswitch is turned off. When you want it to be on and start to express the target gene, you have to add the theophylline to open the riboswitch. Thus we achieved the tightly control of this promoter via a riboswitch. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3098021 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3098021 SequenceAndFeatures</partinfo> | ||
Revision as of 03:32, 17 October 2019
Omp Promoter + riboswitch/sfGFP
The promoter pOmpC is positively regulated, OmpR-controlled promoter (from Part: BBa_RB0082 ). This promoter is taken from the upstream region of OmpC. Phosphorylated OmpR binds to the three operator sites and activates transcription. Its expression is related to the osmotic pressure. In nature, this promoter is upstream of the OmpC porin gene. The regulation of OmpC is determined by the EnvZ-OmpR osmosensing machinery. EnvZ phosphorylates OmpR to OmpR-P. At high osmolarity, EnvZ is more active, creating more OmpR-P. OmpR-P then binds to the low-affinity OmpR operator sites upstream of ompC.
But we found that, this promoter can be used not only for its osmosensing function, in some other condition, it is also be used as a part of a system, such as the red light system ( from Part: BBa_K1017301). In such condition, we hope to get rid of the influence of the osmosis, thus, we create a new part by adding a riboswitch after the promoter, to realize another kind of control via theophylline.
Usage and Biology
This part better to be expressed in DH5α, because the EnvZ-OmpR is a protein naturally expressed in K-12 strain. And the unprocessed state of this part is off, because when there are id no theophylline, the riboswitch is turned off. When you want it to be on and start to express the target gene, you have to add the theophylline to open the riboswitch. Thus we achieved the tightly control of this promoter via a riboswitch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 822