Difference between revisions of "Part:BBa K3292001:Experience"

Latest revision as of 03:10, 17 October 2019

In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzyme comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.

Applications of BBa_K3292001

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-In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.
+In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzyme comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.
 ===Applications of BBa_K3292001===