Difference between revisions of "Part:BBa K3292001:Experience"
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| − | + | In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.<BR>Please enter | |
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
Revision as of 03:09, 17 October 2019
In our case, we decided to add the coding sequence of sialidase from M. viridifaciens. The CDS of this enzymes comes from Micromonospora viridifaciens and was optimized for its expression in E. coli BL21 cells. Encoded by the gen ned A, this enzyme releases sialic acids (N-acetylneuraminic acid) moieties from glycoproteins and glycolipids for use as carbon and energy sources due to its hydrolytic activity.
Please enter
how you used this part and how it worked out.
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