Difference between revisions of "Part:BBa K2960009:Design"
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The mlrD gene sequence has been codon-optimized for expression in E. coli. | The mlrD gene sequence has been codon-optimized for expression in E. coli. | ||
| − | + | The construct was designed to be RFC[10] compatible. | |
===Source=== | ===Source=== | ||
Revision as of 01:31, 17 October 2019
The mlrD transporter protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 126
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 310
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mlrD gene sequence has been codon-optimized for expression in E. coli.
The construct was designed to be RFC[10] compatible.
Source
Sphingopyxis sp. genomic sequence. European Nucleotide Archive. (2019 April 5). Received from https://www.ebi.ac.uk/ena/data/view/QCT05772