Difference between revisions of "Part:BBa K2960010:Design"
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The mlrE gene sequence has been codon-optimized for expression in E. coli. | The mlrE gene sequence has been codon-optimized for expression in E. coli. | ||
| − | + | The construct was designed to be RFC[10] compatible. | |
===Source=== | ===Source=== | ||
Revision as of 01:30, 17 October 2019
The mlrE enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1125
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 84
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mlrE gene sequence has been codon-optimized for expression in E. coli.
The construct was designed to be RFC[10] compatible.
Source
Sphingopyxis sp. genomic sequence. Jin H, Nishizawa T, Guo Y, Nishizawa A, Park H-D, Kato H, Tsuji K, Harada K-I. 2018. Complete genome sequence of a microcystin-degrading bacterium, Sphingosinicella microcystinivorans strain B-9. Microbiol Resour Announc 7:e00898-18. https://doi.org/10.1128/MRA.00898-18.