Difference between revisions of "Part:BBa K3292001:Design"

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<partinfo>BBa_K3292001 short</partinfo>
 
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<partinfo>BBa_K3292001 SequenceAndFeatures</partinfo>
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<partinfo>Bba_J34801 SequenceAndFeatures</partinfo>
  
  

Latest revision as of 01:14, 17 October 2019


T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The purification of the whole construct presented some disadvantages when using imidazole.


Source

Promoter Bba_K2406020 RBS Bba_J34801 Terminator Bba_B0010 sfGFP BBa_I746916 The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI))

References