Difference between revisions of "Part:BBa K3292001:Design"

 
Line 14: Line 14:
  
 
Promoter Bba_K2406020
 
Promoter Bba_K2406020
−
RBS  Bba_K2406020
+
RBS  Bba_J34801
 
Terminator Bba_0010
 
Terminator Bba_0010
 
sfGFP BBa_I746916
 
sfGFP BBa_I746916

Revision as of 01:13, 17 October 2019


T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1986
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 646
    Illegal BsaI.rc site found at 510
    Illegal BsaI.rc site found at 1647
    Illegal SapI.rc site found at 2055


Design Notes

The purification of the whole construct presented some disadvantages when using imidazole.


Source

Promoter Bba_K2406020 RBS Bba_J34801 Terminator Bba_0010 sfGFP BBa_I746916 The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI))

References