Difference between revisions of "Part:BBa K2912015"
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The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene | The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene | ||
| − | We transformed the expression vector into HT115 (DE3) E.coli by heat-shock method | + | We transformed the expression vector into HT115 (DE3) E.coli by heat-shock method, and they were cultured in 1 LB liquid medium to exact exponential phase. Afterwards, we started a cell growth curve experiment of our engineering E.coli(illustrated with Fig.1) |
Revision as of 00:50, 17 October 2019
Trp_Lysis gene
Trp_Lysis gene expression can be controlled by the concentration of Tryptophan
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyze as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene
We transformed the expression vector into HT115 (DE3) E.coli by heat-shock method, and they were cultured in 1 LB liquid medium to exact exponential phase. Afterwards, we started a cell growth curve experiment of our engineering E.coli(illustrated with Fig.1)