Difference between revisions of "Part:BBa K143065:Design"

 
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__NOTOC__
 
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<partinfo>BBa_K143065 short</partinfo>
 
<partinfo>BBa_K143065 short</partinfo>
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<partinfo>BBa_K143065 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143065 SequenceAndFeatures</partinfo>
  
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===Design Notes===
  
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===Design Notes===
 
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.
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===Source===
 
===Source===
  
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry
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===References===
 

Latest revision as of 16:38, 9 October 2008

Spectinomycin Resistance Protein (Aad9) - Terminator



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488

Design Notes

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase. The double terminator is the most commonly used registry termiantor.

Source

Aad9 was PCR cloned from the B. subtilis integration vector utilising Pfu DNA polymerase and cloned into a BioBrick iwth the double terminator was taken from the registry