Difference between revisions of "Part:BBa K2919000:Design"

 
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
An inducible promoter was used for expression, so that the bacteria could grow without the toxin being expressed and killing them off.
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A cumate inducible promoter, CuO, was used for expression, so that the bacteria could grow without the toxin being expressed and killing them off.
 
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===Source===
 
===Source===

Latest revision as of 23:57, 16 October 2019


ghoT toxin from E. coli K12


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A cumate inducible promoter, CuO, was used for expression, so that the bacteria could grow without the toxin being expressed and killing them off.

Source

This part comes from the E. coli K12 genomic sequence

References