Difference between revisions of "Part:BBa J63005:Design"

(→‎Design Notes)
(→‎Design Notes)
Line 9: Line 9:
 
This sequence corresponds to the 1457 bp directly upstream of the start codon of the ADH1 gene in S. cerevisiae.
 
This sequence corresponds to the 1457 bp directly upstream of the start codon of the ADH1 gene in S. cerevisiae.
  
−
'''Wroclaw’s 2019 characterization'''
+
<h2>Wroclaw’s 2019 characterization</h2>
 
<h3>Comparison of the ADH1 and TEF promoters activity</h3>   
 
<h3>Comparison of the ADH1 and TEF promoters activity</h3>   
 
<u>''Background''</u>
 
<u>''Background''</u>

Revision as of 22:50, 16 October 2019


yeast ADH1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 180
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 830


Design Notes

This sequence corresponds to the 1457 bp directly upstream of the start codon of the ADH1 gene in S. cerevisiae.

Wroclaw’s 2019 characterization

Comparison of the ADH1 and TEF promoters activity

Background

Source

genomic DNA of ADH1 from S. cerevisiae

References