Difference between revisions of "Part:BBa K3081104:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed. | |
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===Source=== | ===Source=== | ||
Revision as of 22:29, 16 October 2019
scaffold of sgRNA in CRISPR/Cas system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It already contains a terminator for DNA polymerase. But when you use some specific expression systems like T7, an extra corresponding terminator is needed.
Source
E.coli
References
Previous publication: Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183.v