Difference between revisions of "Part:BBa K2558003:Experience"
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<img style="width:50%" src="https://static.igem.org/mediawiki/parts/1/12/DCas9_Bronze_monoplex.png"> | <img style="width:50%" src="https://static.igem.org/mediawiki/parts/1/12/DCas9_Bronze_monoplex.png"> | ||
<figcaption> | <figcaption> | ||
| − | <b>Figure1: Fluorescence Loss Assay. Amount of GFP fluorescence under the induction of dCas9 targeting GFP. The expression of dCas9 is regulated by an IPTG inducible expression system. Sp1 - 3 represent three different spacers targeting GFP or the associated promoter. SpNT represents a non-targeting spacer acting as a control. </ | + | <b>Figure1: Fluorescence Loss Assay. Amount of GFP fluorescence under the induction of dCas9 targeting GFP.</b> <p> The expression of dCas9 is regulated by an IPTG inducible expression system. Sp1 - 3 represent three different spacers targeting GFP or the associated promoter. SpNT represents a non-targeting spacer acting as a control. </p> |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
Revision as of 22:08, 16 October 2019
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dCas9 Fluorescence Loss Assays
The expression of dCas9 is regulated by an IPTG inducible expression system. Sp1 - 3 represent three different spacers targeting GFP or the associated promoter. SpNT represents a non-targeting spacer acting as a control. 
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