Difference between revisions of "Part:BBa K143054"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K143054 short</partinfo>
 
<partinfo>BBa_K143054 short</partinfo>
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Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''.
 
Inducible promoter hyper-spank(<bbpart>BBa_K143015</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''.
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Phyperspank-gsiB can be used to take an input of IPTG and give a '''Ribosomes per second''' (RiPS) output generator.
 
Phyperspank-gsiB can be used to take an input of IPTG and give a '''Ribosomes per second''' (RiPS) output generator.
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IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K143035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter.
 
IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator '''LacI''', (<bbpart>BBa_K143035</bbpart>). This means that LacI must be constitutively expressed in ''B.subtilis'' in order to use the promoter hyper-spank as an inducible promoter.
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'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
 
'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143054 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143054 SequenceAndFeatures</partinfo>
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===References===
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<biblio>
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#1 https://parts.igem.org/Assembly:RBS-CDS_issues
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</biblio>
  
  

Latest revision as of 16:57, 8 October 2008

Promoter hyper-spank and RBS gsiB for B. subtilis


Inducible promoter hyper-spank(BBa_K143015) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.


Phyperspank-gsiB can be used to take an input of IPTG and give a Ribosomes per second (RiPS) output generator.


IPTG does not directly induce the expression of the promoter hyper-spank, but requires the transcriptional regulator LacI, (BBa_K143035). This means that LacI must be constitutively expressed in B.subtilis in order to use the promoter hyper-spank as an inducible promoter.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>