Difference between revisions of "Part:BBa K3117026"

Revision as of 19:36, 16 October 2019

scFv against GPA33 with SpyCatcher codon optimized for CHOs

This part can form an isopeptide bond with its eqivalent SpyTag (BBa_K3117030) which was part of the project of the iGEM team 2019 FAU_Erlangen, and therefore form a bispecific antibody. The outstanding advantage of the so called SpyTag/SpyCatcher system is that you can fuse this small molecules to arbitrary structures and create a binding. This provides a basis for the establishment of large modular systems.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3117026 short</partinfo>
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+This part can form an isopeptide bond with its eqivalent SpyTag (BBa_K3117030) which was part of the project of the iGEM team 2019 FAU_Erlangen, and therefore form a bispecific antibody. The outstanding advantage of the so called SpyTag/SpyCatcher system is that you can fuse this small molecules to arbitrary structures and create a binding. This provides a basis for the establishment of large modular systems.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The part BBa_K3117026 is a fusion protein of an anti-GPA33 single-chain variable fragment (scFv) and SpyCatcher. The sequence contains a C-terminal His-tag (BBa_K3117005) for easy purification. Secretion of the protein is ensured by an Ig leader (BBa_K3117006). When the protein passes the membrane, the leader segment is cleaved of. By connecting the variable regions of the heavy and the light chain of an anti-GPA33 antibody with a short GGGGS (BBa_K3117004) linker, the scFv retains its antigen-binding ability and is much smaller than a conventional antibody. Thus, it is well suited as part of a fusion protein with another effector. The SpyCatcher attached to the scFv belongs to the SpyTag/SpyCatcher system and is one part of the FbaB protein of Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyTag (BBa_K3117030), they bind covalently. This allows our part to be used in a modular manner in combination with other molecules that have the SpyTag. Since the scFv targets GPA33 which is expressed on more than 95% of colon cancers, our part is optimal for specifically directing its fusion partner (e.g. an effector protein) to colon cancer cells.
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