Difference between revisions of "Part:BBa K3117005"
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<partinfo>BBa_K3117005 short</partinfo> | <partinfo>BBa_K3117005 short</partinfo> | ||
| − | The part BBa_K3117005 is a 6xHis tag which can be used for protein purification and for the evidence of marked proteins. It has the characteristic, that it can bind to Ni2+ ions and | + | The part BBa_K3117005 is a 6xHis tag which can be used for protein purification and for the evidence of marked proteins. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns. |
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Revision as of 19:32, 16 October 2019
6xHis-Tag
The part BBa_K3117005 is a 6xHis tag which can be used for protein purification and for the evidence of marked proteins. It has the characteristic, that it can bind to Ni2+ ions and form a chelat-complex. Therefore, the 6xHis tag can be used to extract the protein using Nickel-based affinity columns.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]