Difference between revisions of "Part:BBa K3110042"

 
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<partinfo>BBa_K3110042 short</partinfo>
 
<partinfo>BBa_K3110042 short</partinfo>
  
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lldRO1 and lldRO2 are the operator regions which bind to the regulator element lldR and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can efficiently discriminate cancer vs non-cancer microenvironments.
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lldRO1 and lldRO2 are the operator regions which bind to the regulator element of Lactate Operon (lldR) and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can produce enough sfGFP to be detected.
  
  

Revision as of 18:46, 16 October 2019


lldRO1-J23117-lldRO2 Weak RBS sfGFP

lldRO1 and lldRO2 are the operator regions which bind to the regulator element of Lactate Operon (lldR) and inhibit transcription. Upon binding of L-Lactate to lldR, however, this transcriptional suppression is lost and instead lldR complex with L-Lactate remains bound to lldRO1 acting as a transcriptional activator. By using RBS of different strengths we are trying to find out the optimum construct which will give output (in our case the output is super-folded GFP or sfGFP) that can produce enough sfGFP to be detected.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 173