Difference between revisions of "Part:BBa K3286046"

 
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<partinfo>BBa_K3286046 short</partinfo>
 
<partinfo>BBa_K3286046 short</partinfo>
  
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Monomeric Red fluorescent protein and Green fluorescent protein downstream of bacteriophage lambda PL, PRM and PR promoter (including the operator regions OL and OR and the 5'UTR's).  
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Monomeric Red fluorescent protein (mRFP) and Green fluorescent protein (GFP) downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's).  
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This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting the essential, early promoters.  
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This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.  
  
 
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Revision as of 16:59, 16 October 2019


mRFP-GFP divergent, Bacteriophage Lambda promoters, operators and 5'UTR's

Monomeric Red fluorescent protein (mRFP) and Green fluorescent protein (GFP) downstream of bacteriophage lambda PL, PRM and PR promoter regions (including the operators OL and OR, and the 5'UTR's). This part has been used to asses the potential of bacteriophage lambda inhibition by CRISPRi, by targeting essential early promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 7
    Illegal AgeI site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1723