Difference between revisions of "Promoters/Catalog"

Revision as of 01:07, 8 October 2008

The registry has a large collection of Promoters available. We categorize promoters using two different parameters. First, most promoters are designed for a particular RNA polymerase holoenzyme, e.g E. coli RNA polymerase with σ70, or T7 RNA polymerase. Second, promoters may be either unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible). So for example, if you want express a gene in growing E. coli at a with little expression before hand, you might choose a lacI repressible σ70 promoter.

The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.

	RNA Polymerase
	E. coli		T7 RNA polymerase
	σ⁷⁰	σ^S
Inducible	See X promoters	See X promoters	See X promoters
Constitutive	See X promoters	See X promoters	See X promoters
Repressible	See X promoters	See X promoters	See X promoters
Multiple	See X promoters	See X promoters	See X promoters

//Promoter/Coli/Sigma70/Constitutive
//Promoter/Coli/Sigma70/Repressible
//Promoter/Coli/Sigma70/Inducible
//Promoter/T7/Constitutive
//Promoter/T7/Repressible

Bacterial promoters
- E. coli promoters
  - σ70 promoters
    - Constitutive promoters
    - Repressible promoters
    - Activatable promoters
Eukaryotic promoters
- Yeast promoters
Phage promoters
- T7 promoters
  - Constitutive T7 promoters
  - Regulatable T7 promoters

@@ Line 4: / Line 4: @@
-{|{{Table}} width="100%"
+{|align="center" border="1" cellpadding="0" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" width="100%"
 !colspan="2" rowspan="3"|Regulation
 !colspan="3"|RNA Polymerase
 |-
-|align="center"|colspan="2"|''E. coli''
+|align="center" colspan="2"|''E. coli''
 |align="center"|T7 RNA polymerase
 |-
@@ Line 14: / Line 14: @@
 |align="center"|&sigma;<sup>S</sup>
 |
-|-
+|-style="height:58px"
-|width="50pt" rowspan="3"|stuff
+|rowspan="4" width="50pt" align="center" |[[Image:BCInducibleIcon.png]][[Image:ConstitutiveIcon.png]][[Image:BCRepressibleIcon.png]][[Image:BCMultipleIcon.png]]
 |width="50pt" |Inducible
 |align="center"| See X promoters
 |align="center"| See X promoters
 |align="center"| See X promoters
-|-
+|-style="height:58px"
 |Constitutive
 |align="center"| See X promoters
 |align="center"| See X promoters
 |align="center"| See X promoters
-|-
+|-style="height:58px"
 |Repressible
 |align="center"| See X promoters
 |align="center"| See X promoters
 |align="center"| See X promoters
-|-
+|-style="height:38px"
-|(+/-) image
 |Multiple
 |align="center"| See X promoters