Difference between revisions of "Part:BBa K2936007"
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Form 7.1 The absorbance of different reaction solution at the wavelength of 350 nm<br> | Form 7.1 The absorbance of different reaction solution at the wavelength of 350 nm<br> | ||
https://static.igem.org/mediawiki/parts/5/5a/T--ZJUT-China--igem-part07form.png<br> | https://static.igem.org/mediawiki/parts/5/5a/T--ZJUT-China--igem-part07form.png<br> | ||
| − | https://static.igem.org/mediawiki/parts/4/4e/T--ZJUT-China--igem-1234.png<br> | + | <img src="https://static.igem.org/mediawiki/parts/4/4e/T--ZJUT-China--igem-1234.png" width="500px"><br> |
Figure 7.1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right | Figure 7.1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right | ||
===Analysis=== | ===Analysis=== | ||
Revision as of 16:23, 16 October 2019
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Description
Short description: Reporter gene.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).
Source
E. coli
Design consideration
LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism
Experiment
Form 7.1 The absorbance of different reaction solution at the wavelength of 350 nm
<img src="
" width="500px">
Figure 7.1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right
Analysis
From the figure 7.1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB is 1 to 10 is the most ideal ratio.