Difference between revisions of "Part:BBa K2941001:Design"
 
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===Design Notes===
 
===Design Notes===
 
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<p>We modified the original biobrick <a href="https://parts.igem.org/Part:BBa_K567018">BBa_K567018</a> because the strain we used (<i>Eschericia coli</i> C321.deltaA.exp) lacked the T7(1). T7 RNA polymerase is present in a few <i>E. coli</i> strains, but we did not find any of them specifically reprogrammed to be able to incorporate any noncanonical amino acids in TAG codon(2). Also we decided to add this as a part because the original biobrick we got from the distribution 2019 plate was not what it should have been.</p>
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<p>We modified the original biobrick <a href="https://parts.igem.org/Part:BBa_K567018">BBa_K567018</a> because the strain we used (<i>Eschericia coli</i> C321.deltaA.exp) lacked the T7(1). T7 RNA polymerase is present in a few <i>E. coli</i> strains, but we did not find any of them specifically reprogrammed to be able to incorporate any noncanonical amino acids in TAG codon(2). We added this part to give other teams a reporter that could work in similar settings.</p>
 
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Latest revision as of 16:15, 16 October 2019


GFP-TAG-RFP (improved from BBa_K567018)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1302
    Illegal AgeI site found at 1414
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We modified the original biobrick BBa_K567018 because the strain we used (Eschericia coli C321.deltaA.exp) lacked the T7(1). T7 RNA polymerase is present in a few E. coli strains, but we did not find any of them specifically reprogrammed to be able to incorporate any noncanonical amino acids in TAG codon(2). We added this part to give other teams a reporter that could work in similar settings.


Source

This is modified from the biobrick BBa_K567018.

References

1. Escherichia coli C321.deltaA.exp from Addgene. [html document visited 10/09/2019]

2. New England Biolabs. E. coli expression strains. [html document visited 10/09/2019]