Difference between revisions of "Part:BBa K3187024"
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<partinfo>BBa_K3187024 short</partinfo> | <partinfo>BBa_K3187024 short</partinfo> | ||
| − | 6x His-Tag is used as a tag for detection and as affinity tag in protein purification. Imidazole | + | The 6x His-Tag is used as a tag for detection and as an affinity tag in protein purification. An Imidazole gradient can later be used to elute the tagged protein from a column containing nickel. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:46, 16 October 2019
6x Histidine (His) Tag + TGA Stop Codon
The 6x His-Tag is used as a tag for detection and as an affinity tag in protein purification. An Imidazole gradient can later be used to elute the tagged protein from a column containing nickel. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]