Difference between revisions of "Part:BBa Q04510"
Willie Jin (Talk | contribs) |
Willie Jin (Talk | contribs) |
||
| Line 27: | Line 27: | ||
=ECUST_China 2019 characterization= | =ECUST_China 2019 characterization= | ||
In ECUST_China 2019 characterization, we added the Plac before the CⅠgene and used mRFP as the reporter gene to measure the effectiveness of this part. | In ECUST_China 2019 characterization, we added the Plac before the CⅠgene and used mRFP as the reporter gene to measure the effectiveness of this part. | ||
| + | In the absence of IPTG, CⅠP was constitutively expressed so that the RFP fluorescence could be observed. However, as the concentration of IPTG increasing, Plac was activated to express CⅠprotein which inhibited the transcription of CⅠP ，so the fluorescence of mRFP decreased. | ||
Revision as of 15:34, 16 October 2019
QPI (B0034.C0051.B0015.R0051)
Lambda cI QPI w/ strong RBS
Usage and Biology
Preliminary data indicates that this inverter functions well. [jb, 5/24/04]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
USTC_2009's MEASUREMENT
ECUST_China 2019 characterization
In ECUST_China 2019 characterization, we added the Plac before the CⅠgene and used mRFP as the reporter gene to measure the effectiveness of this part. In the absence of IPTG, CⅠP was constitutively expressed so that the RFP fluorescence could be observed. However, as the concentration of IPTG increasing, Plac was activated to express CⅠprotein which inhibited the transcription of CⅠP ，so the fluorescence of mRFP decreased.