Difference between revisions of "Part:BBa K2936018"

 
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<partinfo>BBa_K2936018 short</partinfo>
 
<partinfo>BBa_K2936018 short</partinfo>
  
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pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. CAT gene express catalase in the pathway, then the culture added with TMB will turns blue.
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pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The catalase encoded by CAT gene could be reacted with TMB to make the culture blue.
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  <img src="https://static.igem.org/mediawiki/parts/7/75/T--ZJUT-China--igem-part18tonglu.jpg" alt=""width="600px">
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:33, 16 October 2019


pFrmR-CAT 

pFrmR is an engineered formaldehyde-inducible promoter, that expresses the genes when the concentration of CHOH in the environment reach a certain level. The catalase encoded by CAT gene could be reacted with TMB to make the culture blue.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1664
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1664
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 907
    Illegal XhoI site found at 1700
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1664
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1664
  • 1000
    COMPATIBLE WITH RFC[1000]