Difference between revisions of "Part:BBa K3264023:Design"
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Revision as of 15:28, 16 October 2019
TALEsp-vioA-vioB-vioC-vioE
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
an C-terminal domain based on the E. australis MiSp1 sequence (VTSG GYGYGTSAAA GAGVAAGSYA GAVNRLSSAE AASRVSSNIA AIASGGASAL PSVISNIYSG VVASGVSSNE ALIQALLELL SALVHVLSSA SIGNVSSVGV DSTLNVVQDS VGQYVG) Also, the linker between the CT and the Rep (repetitive region) is GNS The construct was cloned into a pT7 plasmid and transformed into BL21 (DE3) E. coli bonded to NT-2Rep-CT