Difference between revisions of "Part:BBa K3286042:Design"
(→Source) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | To assess whether | + | To assess whether bacteriophage Lambda could be inhibited by CRISPRi, we required the native promoter with the OL operators and the 5'UTR. |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 15:03, 16 October 2019
Native bacteriophage Lambda PL promoter, OL operators and 5' UTR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To assess whether bacteriophage Lambda could be inhibited by CRISPRi, we required the native promoter with the OL operators and the 5'UTR.
Source
Sequence was obtained from NCBI Reference Sequence: NC_001416.1 and synthetically synthesised in a gBlock.