Difference between revisions of "Part:BBa K2984032"

Revision as of 14:37, 16 October 2019

L1c-PsaD-ARS-PETase-3xHA-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a 3xHA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1769
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1308
Illegal PstI site found at 1769
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 1032
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1769
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1769
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2984032 short</partinfo>
-This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a HA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
+This part is composed of the PsaD promoter (BBa_K2984008), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), a 3xHA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
 <!-- Add more about the biology of this part here