Difference between revisions of "Part:BBa K118016:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) by MABEL. | + | The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) using the mutagenesis by blunt-ended ligation (MABEL) technique. The genomic DNA sequence also contains to EcoRI sites, both of which have been mutated out using MABEL. |
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===Source=== | ===Source=== | ||
Revision as of 21:20, 6 October 2008
glgC16 (glgC with G336D substitution)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 194
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mutation G336D was achieved by the substitution of A for G at position 1076 (codon GGC->GAC) using the mutagenesis by blunt-ended ligation (MABEL) technique. The genomic DNA sequence also contains to EcoRI sites, both of which have been mutated out using MABEL.
Source
Escherichia coli JM109 genomic DNA