Difference between revisions of "Part:BBa K2936007"
Jiangweiyan (Talk | contribs) (→Experimental Results) |
|||
Line 8: | Line 8: | ||
===<h1>Usage and Biology</h1>=== | ===<h1>Usage and Biology</h1>=== | ||
<p> | <p> | ||
− | + | Mechanism: | |
</p> | </p> | ||
− | + | <br> | |
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/8/89/T--ZJUT-China--igem-part07jili.png" alt=""width="600px"> | ||
+ | </html> | ||
<p> | <p> | ||
− | + | Form 1 The ABS350 of reaction solution of bacterial culture and ONPG | |
− | + | <br> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<html> | <html> | ||
− | < img src="https:// | + | <img src="https://static.igem.org/mediawiki/parts/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px"> |
</html> | </html> | ||
+ | <br> | ||
<html> | <html> | ||
− | <img src="https:// | + | <img src="https://static.igem.org/mediawiki/parts/5/57/T--ZJUT-China--igem-part07reaction.jpg" alt=""width="600px"> |
− | + | ||
</html> | </html> | ||
+ | <br> | ||
+ | <b>Fig1.</b> The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to righ | ||
+ | </p> | ||
+ | <p> | ||
+ | From the figure 7 the color of the third group is enough obvious compared to the background, and at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB was designed to be 1:10. | ||
+ | </p> | ||
Revision as of 13:48, 16 October 2019
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Usage and Biology
Mechanism:
Form 1 The ABS350 of reaction solution of bacterial culture and ONPG
Fig1. The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to righ
From the figure 7 the color of the third group is enough obvious compared to the background, and at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB was designed to be 1:10.