Difference between revisions of "Part:BBa K3268000"
Line 3: Line 3:
 
<partinfo>BBa_K3268000 short</partinfo>
 
<partinfo>BBa_K3268000 short</partinfo>
  
−
This part codes for mouse olfactory receptor, which can bind with heptanal.
+
This part codes for mouse olfactory receptor protein, which can bind with heptanal.
 +
<br>
 +
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).
 +
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
 +
<br>
 +
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
 +
<br>
 +
2. The two PCR primers used for this amplification contain HindII and BamHI sites, respectively.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:40, 16 October 2019


mOR103-15

This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004). We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
2. The two PCR primers used for this amplification contain HindII and BamHI sites, respectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 454
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 834