Difference between revisions of "Part:BBa K143058"

 
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<partinfo>BBa_K143058 short</partinfo>
 
<partinfo>BBa_K143058 short</partinfo>
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Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''.
 
Sigma B dependant promoter ctc(<bbpart>BBa_K143010</bbpart>) coupled to the strong Ribosome Binding Site gsiB(<bbpart>BBa_K143020</bbpart>) from ''B. subtilis''.
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In ''B. subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
 
In ''B. subtilis'' endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol<cite>1</cite>. The promoter ctc has been used previously as a read out for the activation of sigma factor B <cite>2</cite>.
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Pctc has been used to take an input of blue light and give a '''Ribosomes per second'''(RiPS) output.
 
Pctc has been used to take an input of blue light and give a '''Ribosomes per second'''(RiPS) output.
 
To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.
 
To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (<bbpart>BBa_K143037</bbpart>) is required.
  
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'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
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'''To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix<cite>3</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.'''  
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
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<partinfo>BBa_K143058 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143058 SequenceAndFeatures</partinfo>
  
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===References===
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<biblio>
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#1 pmid=3100810
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#2 pmid=17575448
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#3 https://parts.igem.org/Assembly:RBS-CDS_issues
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</biblio>
  
 
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Latest revision as of 16:42, 6 October 2008

Promoter ctc and RBS gsiB for B. subtilis


Sigma B dependant promoter ctc(BBa_K143010) coupled to the strong Ribosome Binding Site gsiB(BBa_K143020) from B. subtilis.


In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.


Pctc has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (BBa_K143037) is required.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix3. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

<biblio>

  1. 1 pmid=3100810
  2. 2 pmid=17575448
  3. 3 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>