Difference between revisions of "Part:BBa K143061"

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<partinfo>BBa_K143061 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K143061 Shttps://parts.igem.org/wiki/index.php?title=Part:BBa_K143061&action=editequenceAndFeatures</partinfo>
  
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The sequence of promoter gsiB was obtained from the ''B. subtilis'' genome and published papers<cite>1 2</cite>. RBS-spoVG were obtained from papers<cite>3</cite> and the sequence synthesised by GeneArt
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===References===
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<biblio>
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#1 pmid=3100810
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#2 pmid=17575448
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#3 https://parts.igem.org/Assembly:RBS-CDS_issues
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</biblio>
  
 
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Revision as of 16:12, 6 October 2008

Promoter gsiB and RBS spoVG for B. subtilis


Sigma B dependant promoter gsiB(BBa_K143011) coupled to the strong Ribosome Binding Site spoVG(BBa_K143021) from B. subtilis.


In B. subtilis endogenous sigma factor B is activated under mild stress. These mild stress conditions can be generally split into nutrient stress response and physical stress response. Nutrient stress response is triggered by low levels of ATP and GTP and physical stress response is triggered by exposure to blue light, salt, heat, acid or ethanol1. The promoter ctc has been used previously as a read out for the activation of sigma factor B 2.


PgsiB has been used to take an input of blue light and give a Ribosomes per second(RiPS) output. To work as a blue light receiver correctly, over-expression of the blue light receptor YtvA (BBa_K143037) is required.


To get the highest level of translation from this Promoter-RBS combination it must be connected to a coding region preceded by a coding region prefix1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features


BBa_K143061 Shttps://parts.igem.org/wiki/index.php?title=Part:BBa_K143061&action=editequenceAndFeatures Not understood

References

<biblio>

  1. 1 pmid=3100810
  2. 2 pmid=17575448
  3. 3 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>